The Ansidei Madonna is a 1505 painting by the Italian High Renaissance artist Raphael. It shows the Blessed Virgin Mary sitting on a wooden throne, with the child Christ on her lap. On her right John the Baptist stands, on her left Saint Nicholas is reading. The painting was originally made for the private chapel of the Ansidei family in the church San Fiorenzo dei Serviti in Perugia. It is now part of the collection of the National Gallery, London.